Platelet-Activating Factor Acetylhydrolase IB Subunit alpha (PAFAH1B1) antibody

Antigen: Platelet-Activating Factor Acetylhydrolase IB Subunit alpha (PAFAH1B1)

Synonyms: MDS, LIS1, LIS2, MDCR, PAFAH

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184751

Additional Information

Alternative name: LIS1 / PAFAH1B1

Gene ID: 5048

Immunogen: TGSVDQTVKVWECR

Format: Affinity-purified

Description: All names and symbols: PAFAH1B1, LIS1, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, MDCR, PAFAH, lissencephaly 1 protein, Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD). Official Symbol: PAFAH1B1. RefSeq number(s): NP_000421. Gene ontology terms: lipid metabolism, neurogenesis, cell motility, signal transduction, cytoplasm, 2-acetyl-1-alkylglycerophosphocholine esterase activity, hydrolase activity

Specificity: Human, Mouse, Rat, Dog 410 aa

Application Details

Application Notes: Western blot: Approx 45kDa and 33kDa bands seen in NIH-3T3 lysate. Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184751 staining (2µg/ml) of NIH-3T3 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Reiner, Bar-Am, Sapir et al.: "LIS2, gene and pseudogene, homologous to LIS1 (lissencephaly 1), located on the short and long arms of chromosome 2." in: Genomics, Vol. 30, Issue 2, pp. 251-6, 1996 (PubMed).