Helicase-Like Transcription Factor (HLTF) antibody

Antigen: Helicase-Like Transcription Factor (HLTF)

Synonyms: Snf2l3, Smarca3, smarca3, wu:fc33f07, HLTF, SMARCA3, MGC131155, RUSH, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, P113, AF010600, BC057116

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184752

Additional Information

Alternative name: SMARCA3

Gene ID: 6596, 20585 (mouse)

Immunogen: C-KQAKINEIRTLIDL

Format: Affinity-purified

Description: All names and symbols: SMARCA3, HLTF, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3, SNF2-like 3, sucrose nonfermenting-like 3, helicase-like transcription factor, DNA-binding protein/plasminogen activator inhibitor-1 regulator. Official Symbol: SMARCA3. RefSeq number(s): NP_003062.2, NP_620636.1. Gene ontology terms: ATP binding, DNA binding, regulation of transcription, DNA-dependent, nucleus, ATP dependent helicase activity, hydrolase activity

Specificity: NP_003062.2 and NP_620636.2 are varients that represent the same protein. Human, Mouse 1009 aa, 1009 aa

Application Details

Application Notes: Western blot: Approx 120kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1µg/ml. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3,270(9):4575-87).

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Transcription Factors, Proteolysis / Ubiquitin

Restrictions: For Research Use only

Images

ABIN184752 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Sheridan, Schorpp, Voz et al.: "Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter." in: The Journal of biological chemistry, Vol. 270, Issue 9, pp. 4575-87, 1995 (PubMed).