Flotillin 2 (FLOT2) (C-Term) antibody

Antigen: Flotillin 2 (FLOT2)

Synonyms: ESA, ECS1, ESA1, ECS-1, M17S1, Esa, AI573412, CG11547, CG14409, FLO2, FLO[Dm]-2, DmelCG32593, CG32593, MGC93331, flot2, MGC52961, FLOT2

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184755

Additional Information

Alternative name: Flotillin 2 / FLOT2 (C Term)

Gene ID: 2319

Immunogen: C-SKIPLIKKATGVQV

Format: Affinity-purified

Description: All names and symbols: FLOT2, flotillin 2, reggie-1, ESA, ESA1, M17S1, Flotillin 2 (epidermal surface antigen 1). Official Symbol: FLOT2. RefSeq number(s): NP_004466.2. Gene ontology terms: cell adhesion, cell adhesion, plasma membrane, epidermal differentiation

Specificity: Human, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum), Lung and Testis lysates (calculated MW of 47.1kDa according to NP_004466.2). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184755 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Rajendran, Masilamani, Solomon et al.: "Asymmetric localization of flotillins/reggies in preassembled platforms confers inherent polarity to hematopoietic cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 14, pp. 8241-6, 2003 (PubMed).