Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1)

Synonyms: HPK1, Hpk1, mHPK1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184758

Additional Information

Alternative name: HPK1 / MAP4K1

Gene ID: 11184

Immunogen: DVVDPDIFNRDPR-C

Format: Affinity-purified

Description: All names and symbols: MAP4K1, mitogen-activated protein kinase kinase kinase kinase 1, HPK1. Official Symbol: MAP4K1. RefSeq number(s): NP_009112. Gene ontology terms: protein kinase activity, activation of JUN kinase, response to stress, protein amino acid phosphorylation

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1/32,000. Western Blot: Approx 95-100kDa band observed in Jurkat (predicted MW of 96kDa according to NP_009112). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184758 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Hu, Qiu, Wang et al.: "Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade." in: Genes & development, Vol. 10, Issue 18, pp. 2251-64, 1996 (PubMed).