Kruppel-Like Factor 8 (KLF8) antibody

Antigen: Kruppel-Like Factor 8 (KLF8)

Synonyms: BKLF3, ZNF741, MGC138314, DKFZp686O08126, ZNF74, A830097P10Rik, klf8, KLF8

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184763

Additional Information

Alternative name: KLF8 / BKLF3

Gene ID: 11279

Immunogen: VDMDKLINNLEVQ-C

Format: Affinity-purified

Description: All names and symbols: KLF8, BKLF3, Kruppel-like factor 8, RP13-1021K9.1, DKFZp686O08126, DXS741, MGC138314, ZNF741, zinc finger protein 741. Official Symbol: KLF8. RefSeq number(s): NP_009181.2. Gene ontology terms: DNA binding, regulation of transcription, DNA-dependent, nucleus

Specificity: Human 359 aa

Application Details

Application Notes: Western blot: Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2) . In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al . J Biol Chem. 2005 Feb 25,280(8):6802-15, PMID: 15557280). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184763 staining (2µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: van Vliet, Turner, Crossley: "Human Kr" in: Nucleic acids research, Vol. 28, Issue 9, pp. 1955-62, 2000 (PubMed).