Kinesin Family Member 5B (KIF5B) antibody

Antigen: Kinesin Family Member 5B (KIF5B)

Synonyms: KNS, KINH, KNS1, UKHC, Khc, KIF5A, DKFZp459M0928

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184774

Additional Information

Alternative name: Kinesin 1 / UKHC

Gene ID: 3799

Immunogen: C-QPVAVRGGGGKQV

Description: All names and symbols: UKHC, kinesin 1 (110-120kD), KIF5B, kinesin family member 5B, KNS, KINH, KNS1, U-KHC. Official Symbol: KIF5B. RefSeq number(s): NP_004512.1. Gene ontology terms: kinesin, microtubule motor, adenosinetriphosphatase, microtubule-based movement

Specificity: Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 120kDa band observed in Human Kidney lysates (calculated MW of 110kDa according to NP_004512.1). Recommended for use at 0.2-1µg/ml. Immunohistochemistry: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration, 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184774 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN184774 (1µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Semiz, Park, Nicoloro et al.: "Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules." in: The EMBO journal, Vol. 22, Issue 10, pp. 2387-99, 2003 (PubMed).