Polynucleotide Kinase 3'-Phosphatase (PNKP) antibody

Antigen: Polynucleotide Kinase 3'-Phosphatase (PNKP)

Synonyms: PNK, MGC94448, PNKP, Tb06.28P18.320, 21.m03011

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184785

Additional Information

Alternative name: PNKP

Gene ID: 11284

Immunogen: EPRLGRLYCQFSEG

Description: All names and symbols: PNKP, polynucleotide kinase 3'-phosphatase, PNK, polynucleotide kinase 3-prime-phosphatase. Official Symbol: PNKP. RefSeq number(s): NP_009185. Gene ontology terms: DNA repair enzyme, response to DNA damage stimulus, DNA repair, nucleus

Specificity: Human 521 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Sucessfully used to detect recombinant human PNKP (1/1000 dilution).

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Jilani, Ramotar, Slack et al.: "Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24176-86, 1999 (PubMed).