Spliceosome-Associated Protein 49 (SF3B4) antibody

Antigen: Spliceosome-Associated Protein 49 (SF3B4)

Synonyms: SAP49, SF3b49, MGC10828

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184797

Additional Information

Alternative name: SF3B4 / SAP49

Gene ID: 10262, 107701 (mouse)

Immunogen: AAGPISERNQDAT-C

Format: Affinity-purified

Description: All names and symbols: SF3B4, SAP49, splicing factor 3b, subunit 4, 49kD, SF3B49, MGC10828, spliceosome-associated protein (U2 snRNP), spliceosomal protein, splicing factor 3b, subunit 4. Official Symbol: SF3B4. RefSeq number(s): NP_005841.1. Gene ontology terms: nucleus, spliceosome, mRNA splicing, mRNA processing, pre-mRNA splicing factor

Specificity: Human, Mouse, Rat 424 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 48kDa band observed in Human HeLa lysates (calculated MW of 44.4kDa according to NP005841.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184797 staining (0.1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Champion-Arnaud, Reed: "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site." in: Genes & development, Vol. 8, Issue 16, pp. 1974-83, 1994 (PubMed).