SF3B3 antibody (C-Term)

Catalog No. ABIN184798

This Goat Polyclonal antibody specifically detects SF3B3 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for SF3B3 antibody (C-Term) (ABIN184798)

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SF3B3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SF3B3 Antibody

Binding Specificity: C-Term

Purpose: SAP130 / SF3B3

Sequence: VSKKLEDIRT RYAF

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-VSKKLEDIRTRYAF, from the C Terminus of the protein sequence according to NP_036558.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136 kDa according to NP_036558.3). Recommended concentration: 0.1-0.3 μg/mL. Preliminary testing was unsuccessful on NIH3T3 for this particular batch. Primary

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SF3B3

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Alternative Name: SF3B3

Background: SF3B3, SAP130, splicing factor 3b, subunit 3, 130 kDa, RSE1, SF3B130, KIAA0017, STAF130, pre-mRNA splicing factor SF3b, 130 kDa subunit, spliceosome-associated protein 130, splicing factor 3b, subunit 3, SF3b130

Gene ID: 23450, 101943

NCBI Accession: NP_036558