Chromatin Licensing and DNA Replication Factor 1 (CDT1) antibody

Antigen: Chromatin Licensing and DNA Replication Factor 1 (CDT1)

Synonyms: DUP, RIS2, Ris2, C76791, AW545653, 2610318F11Rik, cdt1, MGC79890, CDT1, wu:fi38h09, xcdt1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184819

Additional Information

Alternative name: CDT1 / Dup

Gene ID: 81620

Immunogen: C-ARLAHQTRAEEGL

Format: Affinity-purified

Description: All names and symbols: CDT1, DUP, chromatin licensing and DNA replication factor 1, RIS2, Double parked, DNA replication factor Doubkle parked, Drosophila, homolog of. Official Symbol: CDT1. RefSeq number(s): NP_112190.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 65kDa band observed in Human Brain lysates (predicted MW of 63kDa according to NP_112190.1). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184819 staining (1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Whittaker, Royzman, Orr-Weaver: "Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts." in: Genes & development, Vol. 14, Issue 14, pp. 1765-76, 2000 (PubMed).