MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) antibody
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| Synonyms |
MAD2, HSMAD2, AA673185, MGC113763, MAD2L1, cb408, zgc:112133, mad2, XMad2 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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| Catalog no. |
ABIN184822 |
| Quantity |
100 ug |
| Price |
374.27 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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MAD2L1
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Gene ID
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4085, 56150 (mouse)
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Immunogen
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C-KVNSMVAYKIPVND
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Description
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All names and symbols: MAD2L1, MAD2 mitotic arrest deficient-like 1 (yeast), MAD2, HSMAD2, mitotic arrest deficient, yeast, homolog-like 1, MAD2 (mitotic arrest deficient, yeast, homolog)-like 1. Official Symbol: MAD2L1. RefSeq number(s): NP_002349. Gene ontology terms: mitosis, kinetochore, mitotic checkpoint
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Specificity
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Human, Mouse
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Application Notes
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Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Preliminary experiments gave an approx 30kDa band in H460 and Human Brain lysates at 0.2ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 24kDda according to NP_002349. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Cell Cycle
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Restrictions
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For Research Use only
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Li, Benezra: "Identification of a human mitotic checkpoint gene: hsMAD2." in: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 246-8, 1996 (PubMed).
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Alternatives for antigen "MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1)", type "Antibodies"
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Hosts
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Rabbit (44), Mouse (15), Goat (2)
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Reactivities
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Human (60), Mouse (Murine) (25), Rat (Rattus) (24), Chicken (21), Cow (Bovine) (20), Horse (Equine) (18), Pig (Porcine) (18), Dog (Canine) (2), Cat (Feline) (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (44), ELISA (24), Immunofluorescence (IF) (22), Immunoprecipitation (IP) (17), Immunohistochemistry (IHC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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Internal Region (7), AA 100-150 (1), AA 3-13 (1), C-Term (1), Center (1), Internal Region,AA 91-121 (1)
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Alternatives 
