Ribosomal Protein S27 (RPS27) antibody

Antigen: Ribosomal Protein S27 (RPS27)

Synonyms: MPS1, MPS-1, 3200001M24Rik, RPS27, MGC137064, RPS27L

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184825

Additional Information

Alternative name: MPS1 / RPS27

Gene ID: 6232, 57294 (mouse), 94266 (rat)

Immunogen: PLAKDLLHPSPEE-C

Format: Affinity-purified

Description: All names and symbols: MPS1, RPS27, ribosomal protein S27 (metallopanstimulin 1), MPS-1, metallopanstimulin 1, 40S ribosomal protein S27. Official Symbol: RPS27. RefSeq number(s): NP_001021.1. Gene ontology terms: structural constituent of ribosome, RNA binding, zinc ion binding, protein biosynthesis, cell proliferation, signal transduction, ribosome, cytosolic small ribosomal subunit (sensu Eukarya), intracellular

Specificity: Human, Mouse, Rat 84 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 12kDa band observed in lysates of T-cell line Jurkat (calculated MW of 9.5kDa according to NP_001021.1). Recommended concentration: 0.05-0.2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184825 (0.05µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Fernandez-Pol, Klos, Hamilton: "A growth factor-inducible gene encodes a novel nuclear protein with zinc finger structure." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21198-204, 1993 (PubMed).