RPS27 antibody (AA 2-84)

Catalog No. ABIN4886714

Product Details anti-RPS27 Antibody

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Binding Specificity: AA 2-84

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for 40S ribosomal protein S27(RPS27) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for 40S ribosomal protein S27(RPS27) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: ribosomal protein S27
Protein Name: 40S ribosomal protein S27

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MPS1 recombinant protein (Position: P2-H84). Human MPS1 shares 100% amino acid (aa) sequence identity with both mouse and rat MPS1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for RPS27

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Alternative Name: RPS27 (RPS27 Products)

Synonyms: MPS-1 antibody, MPS1 antibody, S27 antibody, 3200001M24Rik antibody, GB13072 antibody, DDBDRAFT_0203425 antibody, DDBDRAFT_0231065 antibody, DDB_0203425 antibody, DDB_0231065 antibody, mps-1 antibody, mps1 antibody, ribosomal protein S27 antibody, 40S ribosomal protein S27 antibody, ribosomal protein S27 pseudogene antibody, Ribosomal protein S27, component of cytosolic 80S ribosome and 40S small subunit antibody, component of 80S ribosome and 40S small subunit antibody, ribosomal protein S27 L homeolog antibody, RPS27 antibody, Rps27 antibody, LOC726295 antibody, RPS27P antibody, rps27 antibody, rps27.L antibody, rps-27 antibody

Background: 40S ribosomal protein S27, also known as metallopan-stimulin 1 or MPS-1, is a protein that in humans is encoded by the RPS27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Synonyms: Metallopan stimulin 1 | Metallopan-stimulin 1 | Metallopanstimulin 1 | Metallopanstimulin1 | MPS-1 | MPS1 | MPS 1 | pmn | rps27 | S27 | P42677

Gene ID: 6232

UniProt: P42677