BRF2, Subunit of RNA Polymerase III Transcription Initiation Factor, BRF1-Like (BRF2) antibody

Antigen: BRF2, Subunit of RNA Polymerase III Transcription Initiation Factor, BRF1-Like (BRF2)

Synonyms: BRFU, FLJ11052, TFIIIB50, AU018504, AU022602, 2700059M06Rik, 5730512K07Rik, BRF2, ZFP36L2, MGC137964

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184848

Additional Information

Alternative name: BRF2

Gene ID: 55290

Immunogen: C-QAARQAATSVPNPP

Format: Affinity-purified

Description: All names and symbols: BRF2, BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like, BRFU, FLJ11052, TFIIIB50, transcription factor IIB- related factor, TFIIIB50, RNA polymerase III transcription initiation factor BRFU. Official Symbol: BRF2. RefSeq number(s): NP_060780. Gene ontology terms: translation initiation factor activity,

Specificity: Human 419 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 50kDa band observed in Hela and Human Placenta lysates (predicted MW of 48kDa according to NP_060780). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN184848 staining (1.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Schramm, Pendergrast, Sun et al.: "Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters." in: Genes & development, Vol. 14, Issue 20, pp. 2650-63, 2000 (PubMed).