Heat Shock 22kDa Protein 8 (HSPB8) antibody

Antigen: Heat Shock 22kDa Protein 8 (HSPB8)

Synonyms: H11, HMN2, CMT2L, DHMN2, E2IG1, HMN2A, HSP22, MGC64408, HSPB8, DKFZp468F234, fc09c11, MGC64202, zgc:64202, wu:fc04b04, wu:fc09c11

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184852

Additional Information

Alternative name: H11 / HSP22

Gene ID: 26353

Immunogen: NELPQDSQEVTCT

Description: All names and symbols: HSPB8, heat shock 22kDa protein 8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, Charcot-Marie-Tooth disease, spinal, heat shock 27kDa protein 8, hereditary motor neuropathy, distal, protein kinase H11, small stress protein-like protein HSP22, spinal muscular atrophy, distal, adult, autosomal dominant. Official Symbol: HSPB8. RefSeq number(s): NP_055180.1. Gene ontology terms: protein serine/threonine kinase activity, heat shock protein activity, biological_process unknown, cellular_component unknown, transferase activity

Specificity: Human 196 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 20-25kDa band seen in human skeletal muscle lysate [Predicted MW of approx. 22kDa according to NP_055180]. Recommended for use at 0.5-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184852 staining (1µg/ml) of human muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Benndorf, Sun, Gilmont et al.: "HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27)." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 26753-61, 2001 (PubMed).