Mitochondrial Ribosomal Protein L3 (MRPL3) antibody

Antigen: Mitochondrial Ribosomal Protein L3 (MRPL3)

Synonyms: MRL3, RPML3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184861

Additional Information

Alternative name: MRPL3

Gene ID: 11222

Immunogen: NVCQPGAPSITFA

Format: Affinity-purified

Description: All names and symbols: MRPL3, mitochondrial ribosomal protein L3, MRL3, RPML3, ribosomal protein, mitochondrial, L3, mitochondrial 60S ribosomal protein L3. Official Symbol: MRPL3. RefSeq number(s): NP_009139.1. Gene ontology terms: RNA binding, protein biosynthesis, mitochondrial large ribosomal-subunit

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 38.6kDa according to NP_009139.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

ABIN184861 staining (1µg/ml) of HepG2 cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Ou, Yen, Wang et al.: "Cloning and characterization of a human ribosomal protein gene with enhanced expression in fetal and neoplastic cells." in: Nucleic acids research, Vol. 15, Issue 21, pp. 8919-34, 1988 (PubMed).