MRPL3 antibody (C-Term)

Catalog No. ABIN2856557

The Rabbit Polyclonal anti-MRPL3 antibody is suitable to detect MRPL3 in samples from Human. It has been validated for WB.

Quick Overview for MRPL3 antibody (C-Term) (ABIN2856557)

Target: MRPL3 (Mitochondrial Ribosomal Protein L3 (MRPL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL3 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to MRPL3 (mitochondrial ribosomal protein L3)
MRPL3 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MRPL3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MRPL3

Target: MRPL3 (Mitochondrial Ribosomal Protein L3 (MRPL3))

Alternative Name: mitochondrial ribosomal protein L3

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q.

Cellular Localization: Mitochondrion

Molecular Weight: 39 kDa

Gene ID: 11222

UniProt: P09001