N-Myc Downstream Regulated 1 (NDRG1) antibody

Antigen: N-Myc Downstream Regulated 1 (NDRG1)

Synonyms: GC4, RTP, DRG1, NDR1, NMSL, TDD5, CAP43, CMT4D, HMSNL, RIT42, TARG1, PROXY1, NDRG1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184872

Additional Information

Alternative name: NDRG1

Gene ID: 10397, 17990 (mouse)

NCBI Acession: NP_006087.2

Immunogen: Peptide with sequence GNSAGPKSMEVSC, from the C Terminus of the protein sequence according to NP_006087.2, NP_001128714.1.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Format: Liquid

Description: Principal Names: tunicamycin-responsive protein, reducing agents and tunicamycin-responsive protein, protein regulated by oxygen-1, nickel-specific induction protein Cap43, differentiation-related gene 1 protein, N-myc downstream regulated gene 1 protein, TDD5, TARG1, RTP, RIT42, PROXY1, NMSL, NDR1, HMSNL, GC4, DRG1, CMT4D, CAP43,, N-myc downstream regulated gene 1, NDRG1. Official Symbol: NDRG1

Specificity: Human, Mouse 394 aa

Molecular Weight: 42.8kDa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kDa band observed in lysates of Human Brain, Kidney and Testis (calculated MW of 42.8kDa according to NP_006087.2). Recommended concentration: 0.1-0.3µg/ml. A minor band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide. IHC: In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cyrtoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration, 0.5-2µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Angiogenesis, Transcription Factors

Restrictions: For Research Use only

Images

ABIN184872 staining (0.1µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Zhou, Salnikow, Costa: "Cap43, a novel gene specifically induced by Ni2+ compounds." in: Cancer research, Vol. 58, Issue 10, pp. 2182-9, 1998 (PubMed).