SET Domain, Bifurcated 2 (SETDB2) antibody

Antigen: SET Domain, Bifurcated 2 (SETDB2)

Synonyms: CLLD8, CLLL8, KMT1F, C13orf4, DKFZp586I0123, DKFZp761J1217, Clld8, Gm293

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184878

Additional Information

Alternative name: CLLD8 / SETDB2

Gene ID: 83852

Immunogen: GEKNGDAKTFWME-C

Format: Affinity-purified

Description: All names and symbols: SETDB2, CLLD8, SET domain, bifurcated 2, CLLL8 protein. Official Symbol: SETDB2. RefSeq number(s): NP_114121.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 80kDa band observed in human heart lysates (calculated MW of 81.9kDa according to NP_114121.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184878 (0.5µg/ml) staining of human heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Mabuchi, Fujii, Calin et al.: "Cloning and characterization of CLLD6, CLLD7, and CLLD8, novel candidate genes for leukemogenesis at chromosome 13q14, a region commonly deleted in B-cell chronic lymphocytic leukemia." in: Cancer research, Vol. 61, Issue 7, pp. 2870-7, 2001 (PubMed).