ABCB9 antibody (C-Term)

Catalog No. ABIN184886

The Goat Polyclonal anti-ABCB9 antibody has been validated for ELISA and IHC. It is suitable to detect ABCB9 in samples from Human.

Quick Overview for ABCB9 antibody (C-Term) (ABIN184886)

Target: ABCB9 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 9 (ABCB9))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCB9 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ABCB9 Antibody

Binding Specificity: C-Term

Purpose: ABCB9 / TAPL

Sequence: GHNEPVANGS HKA

Specificity: This antibody is expected to recognise both human isoforms of this protein (as represented by NP_062570.1, NP_062571.1and NP_982269.1). Variants (NP_062571.1, NP_982269.1) encode the same isoform.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-GHNEPVANGSHKA, from the C Terminus of the protein sequence according to NP_062570.1, NP_062571.1, NP_982269.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows vesiculate cytoplasm staining of primary spermatocytes. Recommended concentration, 3-5 μg/mL.

Western Blot: Preliminary experiments gave no signal but low background in human brain and liver lysates at up to 1 μg/mL. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ABCB9

Target: ABCB9 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 9 (ABCB9))

Alternative Name: ABCB9

Background: ABCB9, ATP-binding cassette, sub-family B (MDR/TAP), member 9, TAPL, KIAA1520, EST122234

Gene ID: 23457

NCBI Accession: NP_062570, NP_062571, NP_982269

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process