ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 9 (ABCB9) antibody

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 9 (ABCB9)

Synonyms: TAPL, KIAA1520, EST122234, mKIAA1520, Tapl, ABCB9, LOC570148

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN184886

Additional Information

Alternative name: ABCB9 / TAPL

Gene ID: 23457

Immunogen: Peptide with sequence C-GHNEPVANGSHKA, from the C Terminus of the protein sequence according to NP_062570.1, NP_062571.1, NP_982269.1

Description: All names and symbols: ABCB9, ATP-binding cassette, sub-family B (MDR/TAP), member 9, TAPL, KIAA1520, EST122234. Official Symbol: ABCB9. RefSeq number(s): NP_062570.1, NP_062571.1, NP_982269.1. Gene ontology terms: lysosome, ATP binding, transporter, spermatogenesis, small molecule transport, integral membrane protein, ATP-binding cassette (ABC) transporter This antibody is expected to recognise both human isoforms of this protein (as represented by NP_062570.1, NP_062571.1and NP_982269.1). Variants (NP_062571.1, NP_982269.1) encode the same isoform..

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave no signal but low background in human brain and liver lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Testis shows vesiculate cytoplasm staining of primary spermatocytes. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Zhang, Zhang, Liu et al.: "Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23287-94, 2000 (PubMed).