UBE2O antibody (C-Term)

Catalog No. ABIN184896

This anti-UBE2O antibody is a Goat Polyclonal antibody detecting UBE2O in ELISA and IHC. Suitable for Human.

Quick Overview for UBE2O antibody (C-Term) (ABIN184896)

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2O antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-UBE2O Antibody

Binding Specificity: C-Term

Purpose: E2-230K / UBE2O

Sequence: ALLEAGMPEC TEDK

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ALLEAGMPECTEDK, from the C Terminus of the protein sequence according to NP_071349.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Heart shows textured staining in some myocardial fibres. Recommended concentration, 4-6 μg/mL.

Western Blot: Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any resul

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2O

Target: UBE2O (Ubiquitin-Conjugating Enzyme E2O (UBE2O))

Alternative Name: UBE2O

Background: UBE2O, ubiquitin-conjugating enzyme E2O, E2-230K, FLJ12878, KIAA1734

Gene ID: 63893, 217342

NCBI Accession: NP_071349