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Ube2t antibody (C-Term)

This Goat Polyclonal antibody specifically detects Ube2t in WB and ELISA. It exhibits reactivity toward Human.
Catalog No. ABIN184897

Quick Overview for Ube2t antibody (C-Term) (ABIN184897)

Target

See all Ube2t Antibodies
Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Reactivity

  • 39
  • 14
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 35
  • 4
Goat

Clonality

  • 34
  • 5
Polyclonal

Conjugate

  • 23
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Ube2t antibody is un-conjugated

Application

  • 28
  • 23
  • 6
  • 4
  • 3
  • 1
  • 1
Western Blotting (WB), ELISA

Grade

Verified
  • Binding Specificity

    • 8
    • 8
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Purpose

    HSPC150 / UBE2T

    Sequence

    QLVGIEKKFH PDV

    Cross-Reactivity

    Human

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Immunogen

    Peptide with sequence C-QLVGIEKKFHPDV, from the C Terminus of the protein sequence according to NP_054895.1.

    Isotype

    IgG
  • Application Notes

    Western Blot: Approx. 22 kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5 kDa according to NP_054895.1). Recommended concentration: 0.1-0.3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

    Alternative Name

    UBE2T

    Background

    UBE2T, HSPC150, PIG50, HSPC150 protein similar to ubiquitin-conjugating enzyme, ubiquitin conjugating enzyme, ubiquitin-conjugating enzyme E2T (putative), ubiquitin-conjugating enzyme E2T

    Gene ID

    29089

    NCBI Accession

    NP_054895

    Pathways

    Chromatin Binding
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