Chromodomain Protein, Y-Like (CDYL) antibody

Antigen: Chromodomain Protein, Y-Like (CDYL)

Synonyms: CDYL1, MGC131936, DKFZp586C1622

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184904

Additional Information

Alternative name: CDYL

Gene ID: 9425, 12593 (mouse)

Immunogen: C-SMLKYLQRKIDEF

Format: Affinity-purified

Description: All names and symbols: CDYL, chromodomain protein, Y-like, CDYL1, DKFZP586C1622, MGC131936, CDY-like, autosomal, bA620A17.2 (chromodomain protein, Y chromosome-like), chromodomain protein, Y chromosome-like, chromodomain protein, Y chromosome-like, isoform b, testis-specific chromodomain Y-like protein. Official Symbol: CDYL. RefSeq number(s): NP_004815.2, NP_736607.1, NP_736608.1. Gene ontology terms: chromatin binding, catalytic activity, chromatin assembly/disassembly, spermatogenesis, metabolism, nucleus, chromatin

Specificity: This antibody is expected to recognize all three reported isoforms (NP_004815.2, NP_736607.1, NP_736608.1). Human, Mouse, Rat, Dog 598 aa, 544 aa, 309 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 75kDa band observed in Human Testes lysates (calculated MW of 66.5kDa according to NP_004815.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25,99(13):8707-12, PMID: 12072557). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling

Restrictions: For Research Use only

Images

ABIN184904 staining (1µg/ml) of Human Testes lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Lahn, Page: "Retroposition of autosomal mRNA yielded testis-specific gene family on human Y chromosome." in: Nature genetics, Vol. 21, Issue 4, pp. 429-33, 1999 (PubMed).