Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) antibody

Antigen: Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B)

Synonyms: WALp4, FLJ45644, DKFZp434H071, DKFZp762I0516, BC053917, D2Ertd794e, 5830435C13Rik, BAZ2B, LOC100229551

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN184905

Additional Information

Alternative name: BAZ2B

Gene ID: 29994

Immunogen: C-YFEKKWTDTFKVS

Description: All names and symbols: BAZ2B, bromodomain adjacent to zinc finger domain, 2B, DKFZP434H071. Official Symbol: BAZ2B. RefSeq number(s): NP_038478

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave an approx 35kDa band in Testis and Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 221kDa according to NP_038478. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Small Intestine shows primarily nuclear staining in a number of cells of the lamina propria. Recommended concentration, 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN184905 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Jones, Hamana, Nezu et al.: "A novel family of bromodomain genes." in: Genomics, Vol. 63, Issue 1, pp. 40-5, 2000 (PubMed).