TRIM24 antibody (C-Term)

Catalog No. ABIN184911

This anti-TRIM24 antibody is a Goat Polyclonal antibody detecting TRIM24 in ELISA and IHC. Suitable for Human.

Quick Overview for TRIM24 antibody (C-Term) (ABIN184911)

Target: TRIM24 (Tripartite Motif Containing 24 (TRIM24))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM24 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TRIM24 Antibody

Binding Specificity: C-Term

Purpose: TIF1A / TRIM24

Sequence: KRLKSIEERQ LLK

Specificity: This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2.

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRLKSIEERQLLK, from the C Terminus of the protein sequence according to NP_056989.2, NP_003843.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast cancer shows nuclear staining in the distorted lobules. Recommended concentration, 4-6 μg/mL.

Western Blot: Preliminary experiments gave an approx 120 kDa band in human kidney and heart lysates at 1 μg/mL, however prominent bands were also seen at 25 kDa and 45 kDa. Please note that currently we cannot find an explanation in the literature for some o

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM24

Target: TRIM24 (Tripartite Motif Containing 24 (TRIM24))

Alternative Name: TRIM24

Background: TRIM24, TIF1, transcriptional intermediary factor 1, PTC6, TF1A, TIF1A, Tif1a, hTIF1, TIF1ALPHA, transcriptional intermediary factor 1 alpha, tripartite motif protein 24, tripartite motif-containing 24, RNF82

Gene ID: 8805

NCBI Accession: NP_056989, NP_003843