Tripartite Motif Containing 28 (TRIM28) antibody

Antigen: Tripartite Motif Containing 28 (TRIM28)

Synonyms: KAP1, TF1B, RNF96, TIF1B, FLJ29029, TRIM28, MGC130965

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN184913

Additional Information

Alternative name: KAP1 / TRIM28

Gene ID: 10155

Immunogen: C-SSQELSGGPGDGP

Format: Affinity-purified

Description: All names and symbols: TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta. Official Symbol: TRIM28. RefSeq number(s): NP_005753. Gene ontology terms: transcription co-repressor activity, zinc ion binding, transcription factor activity, regulation of transcription from Pol II promoter, nucleus

Specificity: Human, Mouse 835 aa

Application Details

Application Notes: Western Blot: Approx 100-120kDa band seen in HepG2 lysate. Recommended for use at 0.2-1µg/ml (Predicted MW approx 92kDa according to NP_005753). Immunofluorescence: Successfully used in on mouse embryo at the late zygote stage, 5ug/ml (personal communication).

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184913 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Friedman, Fredericks, Jensen et al.: "KAP-1, a novel corepressor for the highly conserved KRAB repression domain." in: Genes & development, Vol. 10, Issue 16, pp. 2067-78, 1996 (PubMed).