Tripartite Motif Containing 28 (TRIM28) antibody
| Antigen |
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| Synonyms |
KAP1, TF1B, RNF96, TIF1B, FLJ29029, TRIM28, MGC130965 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), FITC (2), HRP (2), PE,Cy5.5 (2), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunofluorescence (IF)
Western Blotting (WB) (46), Immunofluorescence (IF) (32), ELISA (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Chromatin Immunoprecipitation (ChiP) (2), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN184913 |
| Quantity |
100 ug (Variants) |
| Price |
374.27 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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KAP1 / TRIM28
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Gene ID
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10155
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Immunogen
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C-SSQELSGGPGDGP
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Description
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All names and symbols: TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta. Official Symbol: TRIM28. RefSeq number(s): NP_005753. Gene ontology terms: transcription co-repressor activity, zinc ion binding, transcription factor activity, regulation of transcription from Pol II promoter, nucleus
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Specificity
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Human, Mouse 835 aa
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Application Notes
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Western Blot: Approx 100-120kDa band seen in HepG2 lysate. Recommended for use at 0.2-1µg/ml (Predicted MW approx 92kDa according to NP_005753). Immunofluorescence: Successfully used in on mouse embryo at the late zygote stage, 5ug/ml (personal communication).
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Friedman, Fredericks, Jensen et al.: "KAP-1, a novel corepressor for the highly conserved KRAB repression domain." in: Genes & development, Vol. 10, Issue 16, pp. 2067-78, 1996 (PubMed).
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Alternatives for antigen "Tripartite Motif Containing 28 (TRIM28)", type "Antibodies"
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Hosts
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Rabbit (49), Mouse (14), Goat (6)
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Reactivities
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Human (67), Mouse (Murine) (31), Rat (Rattus) (31), Cow (Bovine) (3), Dog (Canine) (2), Chicken (1), Xenopus laevis (1), Zebrafish (1)
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Applications
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Western Blotting (WB) (46), Immunofluorescence (IF) (32), ELISA (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Chromatin Immunoprecipitation (ChiP) (2), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), FITC (2), HRP (2), PE,Cy5.5 (2), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (7), pSer824 (5), N-Term (3), AA 1-50 (1), pSer46 (1)
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2013 BioMed Central Research Awards
