KAP1 antibody (AA 699-835)

Catalog No. ABIN3043491

Product Details anti-KAP1 Antibody

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Binding Specificity: AA 699-835

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Transcription intermediary factor 1-beta(TRIM28) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Transcription intermediary factor 1-beta(TRIM28) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: tripartite motif containing 28
Protein Name: Transcription intermediary factor 1-beta

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28 (TRIM28 Products)

Synonyms: KAP1 antibody, RNF96 antibody, TF1B antibody, TIF1B antibody, TRIM28 antibody, kap1 antibody, rnf96 antibody, tf1b antibody, tif1b antibody, AA408787 antibody, KAP-1 antibody, KRIP-1 antibody, Tif1b antibody, Tif1beta antibody, Kap1 antibody, Krip1 antibody, TIF1BETA antibody, tripartite motif containing 28 antibody, tripartite motif containing 28 L homeolog antibody, tripartite motif-containing 28 antibody, TRIM28 antibody, trim28.L antibody, Trim28 antibody

Background: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

Synonyms: E3 SUMO protein ligase TRIM28 antibody|E3 SUMO-protein ligase TRIM28 antibody|FLJ29029 antibody|KAP 1 antibody|KAP-1 antibody|KRAB associated protein 1 antibody|KRAB interacting protein 1 antibody|KRAB-associated protein 1 antibody|KRAB-interacting protein 1 antibody|KRIP 1 antibody|KRIP-1 antibody|KRIP1 antibody|Nuclear corepressor KAP 1 antibody|Nuclear corepressor KAP-1 antibody|RING finger protein 96 antibody|RNF96 antibody|TF1B antibody|TIF1 beta antibody|TIF1-beta antibody|TIF1B antibody|TIF1B_HUMAN antibody| Transcription intermediary factor 1 beta antibody|Transcription intermediary factor 1-beta antibody|Trim28 antibody|Tripartite motif containing 28 antibody|tripartite motif containing protein 28 antibody|Tripartite motif-containing protein 28 antibody

Gene ID: 10155

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus