Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (Drosophila) (ARIH1) antibody

Antigen: Ariadne Homolog, Ubiquitin-Conjugating Enzyme E2 Binding Protein, 1 (Drosophila) (ARIH1)

Synonyms: Ari, Ari1, Hari, Hhari, Uip77, Ubch7bp, AU021774, ARI, HARI, HHARI, UBCH7BP, FLJ20329, FLJ93118, DKFZp686O13120, zgc:66364, wu:fa18d01, ARIH1, MGC148912, ariadne, MGC130861, ari, hari, hhari, ubch7bp

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184915

Additional Information

Alternative name: ARIH1 / HHARI

Gene ID: 25820, 23806 (mouse)

Immunogen: C-HEGYEKDLWEYIED

Format: Affinity-purified

Description: All names and symbols: ARIH1, HHARI, ARI, HARI, UBCH7BP, ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila), ariadne homolog, ariadne, Drosophila, homolog of. Official Symbol: ARIH1. RefSeq number(s): NP_005735. Gene ontology terms: ubiquitin-dependent protein catabolism, kinesin complex, ubiquitin ligase complex, enzyme binding, ubiquitin-like-protein ligase activity

Specificity: Human, Mouse 557 aa

Application Details

Application Notes: Peptide ELISA: Pass > 1/32000. Western Blot: Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184915 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Moynihan, Ardley, Nuber et al.: "The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1." in: The Journal of biological chemistry, Vol. 274, Issue 43, pp. 30963-8, 1999 (PubMed).