ARIH2 antibody (C-Term)

Catalog No. ABIN184916

The Goat Polyclonal anti-ARIH2 antibody has been validated for WB, ELISA and IF. It is suitable to detect ARIH2 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for ARIH2 antibody (C-Term) (ABIN184916)

Target: ARIH2 (protein ariadne-2 homolog (ARIH2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ARIH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ARIH2 Antibody

Binding Specificity: C-Term

Purpose: ARIH2 / TRIAD1

Sequence: EQRRRTLLKD FHDT

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8 kDa according to NP_006312.1) Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60 kDa in lys

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ARIH2 antibody (ABIN184916)

Raz, Buijze, Raz, Verwey, Anvar, Aartsma-Rus, van der Maarel: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." in: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, (2014) (PubMed).

Target Details for ARIH2

Target: ARIH2 (protein ariadne-2 homolog (ARIH2))

Alternative Name: ARIH2

Background: ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2

Gene ID: 10425, 23807

NCBI Accession: NP_006312