Ring Finger Protein 39 (RNF39) antibody

Antigen: Ring Finger Protein 39 (RNF39)

Synonyms: HZF, HZFW, LIRF

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184919

Additional Information

Alternative name: RNF39 / LIRF

Gene ID: 80352, 171387 (rat)

Immunogen: CDPRAPLRIVPAES

Format: Affinity-purified

Description: All names and symbols: RNF39, LIRF, ring finger protein 39, HZFW1, HZFw1 protein, LTP (long-term potentiation) induced RING finger protein. Official Symbol: RNF39. RefSeq number(s): NP_079512.1, NP_739575.1

Specificity: This antibody is expected to recognize 2 of the 3 reported isoforms (NP_079512.1 and NP_739575.1 but not NP_739576.1) Human, Rat 420 aa, 354aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 40kDa band observed in human brain lysates (predicted MWs of 41kDa and 48kDa according to NP_079512.1 and NP_739575.1). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184919 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Matsuo, Asada, Fujitani et al.: "LIRF, a gene induced during hippocampal long-term potentiation as an immediate-early gene, encodes a novel RING finger protein." in: Biochemical and biophysical research communications, Vol. 289, Issue 2, pp. 479-84, 2001 (PubMed).