Ring Finger Protein 2 (RNF2) antibody

Antigen: Ring Finger Protein 2 (RNF2)

Synonyms: BAP1, DING, BAP-1, HIPI3, RING2, RING1B

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184925

Additional Information

Alternative name: RNF2 / dinG

Gene ID: 6045, 19821 (mouse)

Immunogen: C-PMELYYAPTKEHK

Format: Affinity-purified

Description: All names and symbols: RNF2, DING, ring finger protein 2, BAP-1, Bap-1, BAP1, HIPI3, RING1B, RING2. Official Symbol: RNF2. RefSeq number(s): NP_009143.1. Gene ontology terms: protein ubiquitination, ubiquitin ligase complex, ubiquitin-protein ligase activity, zinc ion binding

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx. 37-38kDa band observed in lysates of cell lines of NIH3T3 and K562 (calculated MW of 37.7kDa according to NP_009143.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184925 staining (0.5 µg/ml) of K562 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Tuckfield, Clouston, Wilanowski et al.: "Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors." in: Molecular and cellular biology, Vol. 22, Issue 6, pp. 1936-46, 2002 (PubMed).