Ring Finger Protein 8 (RNF8) antibody

Antigen: Ring Finger Protein 8 (RNF8)

Synonyms: FLJ12013, KIAA0646

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184931

Additional Information

Alternative name: RNF8

Gene ID: 9025

Immunogen: GEPGFFVTGDRAG-C

Format: Affinity-purified

Description: All names and symbols: RNF8, ring finger protein 8, ring finger protein (C3HC4 type) 8, KIAA0646, FLJ12013, C3HC4-type zinc finger protein, OTTHUMP00000039684, UBC13/UEV-interacting ring finger protein,. Official Symbol: RNF8. RefSeq number(s): NP_003949.1, NP_898901.1. Gene ontology terms: ligase activity, protein ubiquitination, ubiquitin ligase complex, ubiquitin-protein ligase activity, zinc ion binding,

Specificity: This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1). Human 485 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 55kDa band observed in Human Placenta and Lung lysates (predicted MW of 56kDa according to NP_003949). Recommended for use at 0.1-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Proteolysis / Ubiquitin, Ion Channels

Restrictions: For Research Use only

Images

ABIN184931 staining (0.1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Seki, Hattori, Sugano et al.: "Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif." in: Journal of human genetics, Vol. 43, Issue 4, pp. 272-4, 1999 (PubMed).