Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1) antibody

Antigen: Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1)

Synonyms: PI31, MGC93913

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184933

Additional Information

Alternative name: PI31 / PSMF1 (Isoform 1)

Gene ID: 9491, 228769 (mouse)

Immunogen: C-DHLPPPGYDDMYL

Format: Affinity-purified

Description: All names and symbols: PSMF1, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), RP4-545L17.1, proteasome inhibitor hP131 subunit, proteasome inhibitor subunit 1. Official Symbol: PSMF1. RefSeq number(s): NP_006805.1, NP_848693.1

Specificity: This antibody is expected to recognize isoform 1 (NP_006805.1 and NP_848693.1) only, not isoform 2 (NP_848694.1). Human, Mouse, Rat, Dog 271 aa, ,

Application Details

Application Notes: Peptide ELISA: Pass 1/32000. Western Blot: Approx 35kDa band seen in Human Kidney lysate [Predicted MW of approx. 30kDa according to NP_006805.1]. Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184933 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: McCutchen-Maloney, Matsuda, Shimbara et al.: "cDNA cloning, expression, and functional characterization of PI31, a proline-rich inhibitor of the proteasome." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18557-65, 2000 (PubMed).