PSMF1 antibody (Middle Region)

Catalog No. ABIN2787963

The Rabbit Polyclonal anti-PSMF1 antibody (ABIN2787963) specifically detects PSMF1 in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Cow, Horse and Guinea Pig samples.

Quick Overview for PSMF1 antibody (Middle Region) (ABIN2787963)

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMF1 Antibody

Binding Specificity: Middle Region

Sequence: DYIDAEHLGD FHRTYKNSEE LRSRIVSGII TPIHEQWEKA NVSSPHREFP

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PSMF1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSMF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 271 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PSMF1

Target: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Alternative Name: PSMF1

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.
Alias Symbols: PI31
Protein Interaction Partner: FBXO7, SHFM1, UBC, BEND7, RHOXF2, KHDRBS3, PAK7, LDOC1, RBFOX2, PSMA7, DVL3, RALYL, RBFOX1, QKI, CD2BP2, CUL1, PSMA2, MIEF2, CCDC85B, RNF126, PDLIM7, HOOK2, NUDT21, CTBP2, TRAF2, MAGEA11, RBMX,
Protein Size: 271

Molecular Weight: 30 kDa

Gene ID: 9491

NCBI Accession: NM_006814, NP_006805

UniProt: Q92530

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA