Ring Finger Protein 22 (TRIM3) antibody

Antigen: Ring Finger Protein 22 (TRIM3)

Synonyms: BERP, HAC1, RNF22, RNF97, FLJ16135

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184941

Additional Information

Alternative name: BERP / RNF22

Gene ID: 10612, 55992 (mouse), 83616 (rat)

Immunogen: AKREDSPGPEVQP-C

Format: Affinity-purified

Description: All names and symbols: BERP, RNF22, TRIM3, tripartite motif-containing 3, HAC1, RNF97, ring finger protein 22, brain expressed ring finger, tripartite motif protein TRIM3. Official Symbol: TRIM3. RefSeq number(s): NP_006449.2, NP_150594.2. Gene ontology terms: cytoplasm, neurogenesis, protein C-terminus binding

Specificity: Both variants (NP_006449.2, NP_150594.2,) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein. Human, Mouse, Rat, Dog 744 aa, 733 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 80kDa band observed in Mouse Brain lysates (calculated MW of 80.8kDa according to NP_006449.2 and NP_150594.2). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184941 staining (1ug/ml) of Mouse Brain (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing TRIM3 (RC211928) and probed with ABIN184941 (mock transfection in first lane), tested by Origene.

Publications

Publications: El-Husseini, Fretier, Vincent: "Cloning and characterization of a gene (RNF22) encoding a novel brain expressed ring finger protein (BERP) that maps to human chromosome 11p15.5." in: Genomics, Vol. 71, Issue 3, pp. 363-7, 2001 (PubMed).