NIF3-Like Protein 1 (NIF3L1) antibody

Antigen: NIF3-Like Protein 1 (NIF3L1)

Synonyms: CALS-7, MDS015, ALS2CR1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184942

Additional Information

Alternative name: ALS2CR1 / NIF3L1

Gene ID: 60491, 65102 (mouse)

Immunogen: Peptide with sequence C-ILSETDRDPLQVV, from the C Terminus of the protein sequence according to NP_001129511.1, NP_068596.2

Format: Affinity-purified

Description: All names and symbols: NIF3L1, ALS2CR1, NIF3 NGG1 interacting factor 3-like 1 (S. pombe), CALS-7, MDS015, NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1, NIF3 NGG1 interacting factor 3-like 1, OTTHUMP00000206190, OTTHUMP00000206191, OTTHUMP00000206192. Official Symbol: NIF3L1. RefSeq number(s): NP_001129511.1, NP_068596.2.

Specificity: Expected from sequence similarity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 40kDa band seen in Human Heart and Human Testis extracts [Predicted MW of approx. 39kDa according to NP_068596]. Recommended for use at 0.2-1µg/ml. Immunohistochemistry: In paraffin embedded Human Breast shows strong cytoplasm staining in epithelial and myoepithelial duct cells. Recommended concentration, 2-4µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: El-Husseini, Fretier, Vincent: "Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2." in: Genomics, Vol. 71, Issue 2, pp. 200-13 (PubMed).