ClpP Caseinolytic Peptidase, ATP-Dependent, Proteolytic Subunit Homolog (E. Coli) (CLPP) antibody

Antigen: ClpP Caseinolytic Peptidase, ATP-Dependent, Proteolytic Subunit Homolog (E. Coli) (CLPP)

Synonyms: CLPP, MGC130667, MGC137504

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184946

Additional Information

Alternative name: CLPP

Gene ID: 8192

Immunogen: Peptide with sequence C-EAAPAAEPVPAST, from the C Terminus of the protein sequence according to NP_006003.1

Format: Affinity-purified

Description: All names and symbols: CLPP, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E. coli), ATP-dependent proteolytic subunit E.coli homolog, ATP-dependent protease ClpAP (E. coli), proteolytic subunit, human, ClpP (caseinolytic protease, ATP-dependent, proteolytic subunit, E. coli) homolog, ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli), ATP-dependent protease ClpAP, proteolytic subunit, human, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog, caseinolytic peptidase, ATP-dependent, proteolytic subunit. Official Symbol: CLPP. RefSeq number(s): NP_006003.1. Gene ontology terms: endopeptidase Clp activity, peptidase activity, proteolysis and peptidolysis.

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 27-30kDa band observed in human heart and skeletal muscle lysates (predicted MW of 32kDa according to NP_006003). Recommended for use at 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Liver shows patterned staining of hepatocyte cytoplasm. Recommended concentration, 2-4µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Publications

Publications: Bross, Andresen, Knudsen et al.: "Human ClpP protease: cDNA sequence, tissue-specific expression and chromosomal assignment of the gene." in: FEBS letters, Vol. 377, Issue 2, pp. 249-52, 1996 (PubMed).