Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member B (ANP32B) antibody

Antigen: Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member B (ANP32B)

Synonyms: APRIL, SSP29, PHAPI2, PAL31, Ssp29, PHAPI2a, MGC103080, 2410015B15Rik, cb908, fa99a11, zgc:77745, wu:fa28c10, wu:fa99a11, wu:fb40e12, anp32b, MGC76086, ANP32B, hLAMP-1, MGC80871, MGC128074, MGC69373

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184956

Additional Information

Alternative name: SSP29 / ANP32B

Gene ID: 10541, 67628 (mouse), 170724 (rat)

Immunogen: C-KRKRETDDEGEDD

Format: Affinity-purified

Description: All names and symbols: SSP29, ANP32B, APRIL, PHAPI2, acidic (leucine-rich) nuclear phosphoprotein 32 family, member B, acidic protein rich in leucines, OTTHUMP00000063770. Official Symbol: ANP32B. RefSeq number(s): NP_006392.1

Specificity: This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. Human, Mouse, Rat 251 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: 30 kDa band observed in A431 and human lymph node lysates (predicted MW 29kDa according to NP_006392). Recommended for use at 0.2-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184956 staining (1ug/ml) of human lymph node lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Zhu, Perlaky, Henning et al.: "Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR family." in: Biochemistry and molecular biology international, Vol. 42, Issue 5, pp. 927-35, 1998 (PubMed).