CARD11 antibody (C-Term)

Catalog No. ABIN184957

This Goat Polyclonal antibody specifically detects CARD11 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CARD11 antibody (C-Term) (ABIN184957)

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CARD11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CARD11 Antibody

Binding Specificity: C-Term

Purpose: CARD11

Sequence: QRKTIWVDED QL

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QRKTIWVDEDQL, from the C Terminus of the protein sequence according to NP_115791.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5 μg/mL.

Western Blot: Approx 150+65 kDa bands observed in lysates of cell line Jurkat (calculated MW of 133 kDa according to NP_115791.2). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 60 kDa. This ba

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CARD11

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Alternative Name: CARD11

Background: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3

Gene ID: 84433, 108723

NCBI Accession: NP_115791

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, BCR Signaling