Caspase Recruitment Domain Family, Member 11 (CARD11) antibody

Antigen: Caspase Recruitment Domain Family, Member 11 (CARD11)

Synonyms: BIMP3, CARMA1, MGC133069, 0610008L17Rik, 2410011D02Rik, CARD11, MGC165904, LOC100232755

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184957

Additional Information

Alternative name: CARD11

Gene ID: 84433, 108723 (mouse)

Immunogen: C-QRKTIWVDEDQL

Description: All names and symbols: CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3. Official Symbol: CARD11. RefSeq number(s): NP_115791.2

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11. These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Apoptosis/Necrosis, Adaptive Immunity

Restrictions: For Research Use only

Images

ABIN184957 staining (1µg/ml) of human Jurkat cells (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Bertin, Wang, Guo et al.: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001 (PubMed).