Sorting Nexin 16 (SNX16) antibody

Antigen: Sorting Nexin 16 (SNX16)

Synonyms: DKFZp666H147, SNX16, Snx16, AI117577, AI747675, 4930522N22Rik, MGC77396, snx16, DKFZp459I061, LOC100230665

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184967

Additional Information

Alternative name: SNX16

Gene ID: 64089, 74718 (mouse), 64088 (rat)

Immunogen: ATPYVPVPMPIGN-C

Format: Affinity-purified

Description: All names and symbols: SNX16, sorting nexin 16, DKFZp666H147. Official Symbol: SNX16. RefSeq number(s): NP_071416.2, NP_690049.1, NP_690050.1. Gene ontology terms: protein transporter activity, intracellular protein transport, intracellular signaling cascade, kinesin complex

Specificity: This antibody is expected to recognise both isoform a (NP_071416.2, NP_690049.1) and isoform b (NP_690050.1). Human, Mouse, Rat, Dog, Cow 344 aa, 344 aa, 315 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.2kDa according to NP_071416.2). Recommended concentration: 0.05-0.2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Transporters

Restrictions: For Research Use only

Images

ABIN184967 (0.05µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Teasdale, Loci, Houghton et al.: "A large family of endosome-localized proteins related to sorting nexin 1." in: The Biochemical journal, Vol. 358, Issue Pt 1, pp. 7-16, 2001 (PubMed).