TRIM2 antibody (C-Term)

Catalog No. ABIN184974

This anti-TRIM2 antibody is a Goat Polyclonal antibody detecting TRIM2 in WB, ELISA, FACS and IHC. Suitable for Human and Rat.

Quick Overview for TRIM2 antibody (C-Term) (ABIN184974)

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TRIM2 Antibody

Binding Specificity: C-Term

Purpose: TRIM2

Sequence: DSGNHCFKVY RYLQ

Specificity: Cross-reactivity with TRIM3 has been demonstrated in Mouse.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2, NP_001123539.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies. Recommended concentration: 6-12 μg/mL.

Western Blot: Approx 75-85 kDa band observed in Rat Brain lysates (calculated MW of 83.3 according to Rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation wi

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM2

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Alternative Name: TRIM2

Background: TRIM2, tripartite motif-containing 2, KIAA0517, tripartite motif protein 2, tripartite motif protein TRIM2, RNF86, tripartite motif protein TRIM2

Gene ID: 23321, 80890

NCBI Accession: NP_056086, NP_001123539, NP_001337983, NP_001337984, NP_001337986