Tripartite Motif Containing 7 (TRIM7) antibody

Antigen: Tripartite Motif Containing 7 (TRIM7)

Synonyms: GNIP, RNF90

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184987

Additional Information

Alternative name: GNIP / TRIM7

Gene ID: 81786

Immunogen: C-QAPAGPPWDITEA

Format: Affinity-purified

Description: All names and symbols: TRIM7, GNIP1, RNF90, tripartite motif-containing 7, tripartite motif protein TRIM7, glycogenin-interacting protein 1. Official Symbol: TRIM7. RefSeq number(s): NP_203128

Specificity: Human 221 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 25-30kDa band observed in Human Skeletal Muscle lysate (predicted MW of 25kDa according to NP_203128). Recommended for use at 1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184987 staining (1µg/ml) of Human Muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Skurat, Dietrich, Zhai et al.: "GNIP, a novel protein that binds and activates glycogenin, the self-glucosylating initiator of glycogen biosynthesis." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19331-8, 2002 (PubMed).