Ubiquitin Specific Peptidase 11 (USP11) antibody

Antigen: Ubiquitin Specific Peptidase 11 (USP11)

Synonyms: UHX1, USP11, MGC6649, KIAA4085, mKIAA4085, 6230415D12Rik, Uhx1, MGC105909, DKFZp459L098

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185009

Additional Information

Alternative name: USP11 / UHX1

Gene ID: 8237

Immunogen: CSSPPSSEFMDVN

Format: Affinity-purified

Description: All names and symbols: USP11, ubiquitin specific protease 11, UHX1, deubiquitinating enzyme 11, ubiquitin thiolesterase 11, ubiquitin carboxyl-terminal hydrolase, X-linked. Official Symbol: USP11. RefSeq number(s): NP_004642

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 120-130kDa band observed in Jurkat and Human Testis lysates (despite a predicted MW of 110 according to NP_004642 this protein has previously been reported to run significantl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185009 staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Ideguchi, Ueda, Tanaka et al.: "Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM." in: The Biochemical journal, Vol. 367, Issue Pt 1, pp. 87-95, 2002 (PubMed).