Myosin VIIA and Rab Interacting Protein (MYRIP) antibody

Antigen: Myosin VIIA and Rab Interacting Protein (MYRIP)

Synonyms: SLAC2C, SLAC2-C, FLJ44025, MGC130034, MGC130035, DKFZp586F1018, Slac2c, Slac2-c, A230081N12Rik, MYRIP, LOC561671, LOC100156583

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185041

Additional Information

Alternative name: MYRIP / SLAC2C

Gene ID: 25924

Immunogen: C-KDLMEPALESAVMY

Format: Affinity-purified

Description: All names and symbols: MYRIP, SLAC2C, myosin VIIA and Rab interacting protein, MyRIP, SLAC2-C, exophilin, exophilin 8, DKFZp586F1018, rab effector MYRIP, Slp homologue lacking C2 domains, synaptotagmin-like protein homologue lacking C2 domains-c, FLJ44025, MGC130034, MGC130035. Official Symbol: MYRIP. RefSeq number(s): NP_056275.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 100kDa band observed in Human Duodenum lysates (calculated MW of 95.7kDa according to NP_056275.2). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185041 staining (0.1µg/ml) of Human Duodenum lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: El-Amraoui, Schonn, Kuessel-Andermann et al.: "MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes." in: EMBO reports, Vol. 3, Issue 5, pp. 463-70, 2002 (PubMed).