RAN Binding Protein 9 (RANBP9) antibody

Antigen: RAN Binding Protein 9 (RANBP9)

Synonyms: RANBPM, Ibap1, IBAP-1, RanBPM, DmelCG5252, CG5252, RANBP9, cb457, zgc:158650, ranbpm, ranbp10, ranbp9a, Ranbpm

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185045

Additional Information

Alternative name: RANBPM / RANBP9

Gene ID: 10048, 56705 (mouse)

Immunogen: GSCAFATVEDYLH

Format: Affinity-purified

Description: All names and symbols: RANBP9, RanBPM, RAN binding protein 9, RANBPM, novel centrosomal protein RanBPM, ran binding protein, centrosomal. Official Symbol: RANBP9. RefSeq number(s): NP_005484.2. Gene ontology terms: RAN protein binding, microtubule nucleation, cell growth and/or maintenance, protein complex assembly, microtubule associated complex

Specificity: This product is also expected to recognize the centrosome-associated truncated isoform RANBPM (BAA23216.1). Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave no signal but low background in whole cell Hela lysates at up to 1µg/ml. Immunohistochemistry: In paraffin embedded human tonsil, stains nuclei in epithelial cells of tonsillar criptae. Recommended concentration, 3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185045 (3µg/ml) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Wang, Li, Messing et al.: "Activation of Ras/Erk pathway by a novel MET-interacting protein RanBPM." in: The Journal of biological chemistry, Vol. 277, Issue 39, pp. 36216-22, 2002 (PubMed).