Forkhead Box K2 (FOXK2) (isoform 1) antibody

Antigen: Forkhead Box K2 (FOXK2)

Synonyms: wu:fc20a11, FOXK2, foxk2, fa28c04, ILF, ILF1, ILF-1

Epitope: isoform 1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185049

Additional Information

Alternative name: FOXK2 / ILF (isoform 1)

Gene ID: 3607

Immunogen: C-TPPAAVREKGVQN

Format: Affinity-purified

Description: All names and symbols: ILF1, FOXK2, ILF, ILF-1, interleukin enhancer binding factor 1, cellular transcription factor ILF-1, forkhead box K2. Official Symbol: FOXK2. RefSeq number(s): NP_004505. Gene ontology terms: RNA polymerase II transcription factor activity, transcription factor activity, regulation of transcription from Pol II promoter, nucleus

Specificity: This antibody is expected to recognise isoform 1 (represented by NP_004505) but not isoforms 2 and 3 (represented by NP_852095 and NP_852096) Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 75-80 kDa band observed in Human Heart extracts (predicted MW of 73kDa according to NP_004505). Recommended for use at 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185049 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Li, Lusis, Sparkes et al.: "Characterization and chromosomal mapping of the gene encoding the cellular DNA binding protein ILF." in: Genomics, Vol. 13, Issue 3, pp. 665-71, 1992 (PubMed).