SMARCE1 antibody (C-Term)

Catalog No. ABIN185062

This Goat Polyclonal antibody specifically detects SMARCE1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for SMARCE1 antibody (C-Term) (ABIN185062)

Target: SMARCE1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SMARCE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SMARCE1 Antibody

Binding Specificity: C-Term

Purpose: BAF57 / SMARCE1

Sequence: PPTDPIPEDE KKE

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PPTDPIPEDEKKE, from the C Terminus of the protein sequence according to NP_003070.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 50 kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6 kDa according to NP_003070.3). A 48 kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SMARCE1

Target: SMARCE1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))

Alternative Name: SMARCE1

Background: SMARCE1, BAF57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, mammalian chromatin remodeling complex BRG1-associated factor 57, BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chro

Gene ID: 6605, 57376

NCBI Accession: NP_003070

Pathways: Chromatin Binding