Forkhead Box N1 (FOXN1) antibody

Antigen: Forkhead Box N1 (FOXN1)

Synonyms: WHN, RONU, FKHL20, HTLF, Fkh19, Foxn1, 3230402J05Rik, 6030465J18Rik, nu, Whn, nude, Hfh11, MGC124316, MGC124317, D11Bhm185e, whnb, Rnu, FOXN1, foxN1, foxN, foxn1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185069

Additional Information

Alternative name: FOXN1

Gene ID: 8456

Immunogen: C-SVYLSPSSKPVALA

Format: Affinity-purified

Description: All names and symbols: FOXN1, WHN, winged-helix nude, FKHL20, forkhead box protein N1. Official Symbol: FOXN1. RefSeq number(s): NP_003584. Gene ontology terms: specific RNA polymerase II transcription factor, epidermal differentiation, histogenesis and organogenesis, defense response, regulation of transcription from Pol II promoter, nucleus

Specificity: Human 648 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: preliminary experiments in Human Thymus lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Thymus Medulla shows speculate nuclear staining in some of the Medullar cells. Recommended concentration, 20-30µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Adaptive Immunity

Restrictions: For Research Use only

Images

ABIN185069 (20µg/ml) staining of paraffin embedded Human Thymus Medulla. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Schlake, Schorpp, Maul-Pavicic et al.: "Forkhead/winged-helix transcription factor Whn regulates hair keratin gene expression: molecular analysis of the nude skin phenotype." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 217, Issue 4, pp. 368-76, 2000 (PubMed).